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KMID : 0620920170490080007
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Experimental & Molecular Medicine
2017 Volume.49 No. 8 p.7 ~ p.7
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SH3BP4, a novel pigmentation gene, is inversely regulated by miR-125b and MITF
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Kim Kyu-Han
Lee Tae-Ryong
Cho Eun-Gyung
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Abstract
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Our previous work has identified miR-125b as a negative regulator of melanogenesis. However, the specific melanogenesis-related genes targeted by this miRNA had not been identified. In this study, we established a screening strategy involving three consecutive analytical approaches?analysis of target genes of miR-125b, expression correlation analysis between each target gene and representative pigmentary genes, and functional analysis of candidate genes related to melanogenesis?to discover melanogenesis-related genes targeted by miR-125b. Through these analyses, we identified SRC homology 3 domain-binding protein 4 (SH3BP4) as a novel pigmentation gene. In addition, by combining bioinformatics analysis and experimental validation, we demonstrated that SH3BP4 is a direct target of miR-125b. Finally, we found that SH3BP4 is transcriptionally regulated by microphthalmia-associated transcription factor as its direct target. These findings provide important insights into the roles of miRNAs and their targets in melanogenesis.
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KEYWORD
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Cellular signalling networks, miRNAs
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